This thesis has been focused around the Diels Alder reaction with the goal to design an enzyme catalyzed reaction pathway. To achieve this goal computer aided enzyme design was utilized. Common traditional methods of computational chemistry (B3LYP, MP2) do not do well when calculating reaction barriers or even reaction energies for the Diels Alder reaction. New calcu- lation methods were developed and tested. This was the focus of the first part of the thesis, by choosing a small system, extensive and heavy calculations could be done with CBS-QB3.

This thesis has been focused around the Diels Alder reaction with the goal to design an enzyme catalyzed reaction pathway. To achieve this goal computer aided enzyme design was utilized. Common traditional methods of computational chemistry (B3LYP, MP2) do not do well when calculating reaction barriers or even reaction energies for the Diels Alder reaction. New calcu- lation methods were developed and tested. This was the focus of the first part of the thesis, by choosing a small system, extensive and heavy calculations could be done with CBS-QB3.

This study investigated the relation between Extraversion and Conscientiousness with job satisfaction and job selection in the two occupational groups of telemarketing and administration. The study also set out to explore the relation between social desirability as assessed with an Implicit Association Test (IAT) and total Reaction time on the IAT. The questionnaire was administered over the Internet to forty-one subjects (24 women and 17 men) in the age range 16-65. Results indicated that people working with telemarketing tend to be extrovert while people working with administration often are conscientious. Job satisfaction correlated with Extraversion in the telemarketing group.

Nuts of all kinds are common ingredients in food. For nut allergy sufferers the frequent use of nuts cause problems and "hidden" nuts in food products may elicit allergic reaction when such foods are consumed. Methods for detecting and quantifying walnut (and other nuts) with high sensitivity and specificity are therefore very important.The objective of this project was to verify the specificity of a rabbit antiserum against walnut with immunodiffusion and to determine the size of the dominant walnut antigens with Western blotting. In addition, a commercial sandwich ELISA for walnut quantification was validated and compared with a qualitative real-time PCR.The rabbit antiserum proved to be less specific but after absorption with cross-reacting nuts and seeds it showed high specificity. The ELISA kit reacted, except for walnut, with pecan and slightly with other nuts and seeds tested.

Dyslexi är en form av läs- och skrivsvårighet som inte kan förklaras av t.ex. bristfällig undervisning, låg begåvning eller synproblem. Orsaken till dyslexi anses vanligen vara nedsatt förmåga av fonologiskt processande. Flera studier har dock visat att dyslexi även är förknippat med språkliga, kognitiva och motoriska svårigheter som inte kan förklaras av nedsatt förmåga av fonologiskt processande. Det procedurella minnessystemet engageras vid inlärning, processande och konsolidering av kognitiva och motoriska färdigheter.

In this study I investigated whether the strength in the manual response for emotional and neutral stimulus search arrays can explain some of the difference in Reaction times (RT:s) for emotional stimuli, or not. Earlier, perceptual, cognitive and attentional processes has mostly been used to explain the variance in RT:s. Thirtythree individuals were asked to, as fast as possible, detect a target picture among distractor pictures in 3 rows x 4 columns arranged search arrays. The pictures were categorized in fear relevant animals (snake, spider) and neutral animals (cat). The results showed, more correct responses for cat target.

The aim of this study was to investigate emotions and emotion regulation of a downsizing in an industrial company. The sample consisted of persons who had experienced a downsizing but have kept the employment, within a same position or being transferred to another one. Data consisted of two focus group interviews, analysed by an inductive thematic analysis. The results have indicated that emotional reactions changed over time as well the emotional regulation did during the different phases. The groups differed in emotional reaction related to the time of decision regarding the retention of employment.

Tissue factor (TF), a 47 kDa glycoprotein, is the initiator of the extrinsic pathway of blood coagulation and consequently of the upmost importance when damage to blood vessel occurs. The source of TF in circulation has been investigated. However, the source of TF is still not clear. One theory is that platelets express and increases the expression of TF after stimulation and the aim of our report was to investigate whether platelets really are a source for TF in circulation.Using specific primers for TF mRNA, platelets in plasma from healthy volunteers and from patients suffering from cardiac infarction were analyzed by using real-time polymerase chain reaction (PCR). Gel electrophoresis was performed after amplification of TF mRNA to verify the results.The samples were negative for TF when using real-time PCR and the few positive all had cycle threshold (Ct) values above 35.

This student report is about canine atopic dermatitis It includes a description of the immunologic mechanisms and how the reaction affects the body. The symptoms are various, most common is pruritus, erythema and alopecia and directly associated with the immunologic reaction. The diagnostic process includes symptoms, physical findings, differential diagnosis, intradermal test and serological test. The options of treatment are several and vary for each patient. The report includes hyposensitization, medical treatment such as corticosteroids, antihistamin and cyclosporine, topical treatment, fatty acid supplements and environmental treatment. The prognosis is individual for each patient due to their different response to the treatment. It may take a while to find the right combination of treatments to achieve satisfactory control of the disease..

The purpose of this study is to examine the capital market reaction generated by layoff announcements. The study takes place in Sweden and tests 138 news concerning layoffs which are categorized according to a content analysis. They are divided as: positive or negative news. The layoffs are also divided into three subgroups with varying sizes: 0,98 percent or less, between 0,99 and 4,49 percent or 4,5 percent or larger. The study uses an event study method to measure the reaction of the capital market.

The aim of this master thesis is to examine which criteria are generally used to guide pub­lic librarians in their acquisitions' decision. The study went ahead in France, principally at La Bibliotheque municipale de Lyon. It also includes a view of some special library services in the social field.Twenty persons were interviewed and an attempt was made to divide their answers into three sections: acquisition routines and criteria for media purchase, how to receive the library visi­tors and their influence on the acquisitions, and, finally, the librarians' own opinions on the matter. One important question was whether censorship is applied and in that case, would it be a deliberately way of acting or rather a spontaneous reaction, more or less unconscious.Censorship in real or manifested through a self-preservative reaction turned out to be a factor closely dependant on the library's financial situation. Many librarians affirmed they would find useful some kind of a written practical library acquisition policy..

Purpose: The purpose of this thesis is to investigate whether abnormal stock returns could be identified as a result of a layoff announcement during weak economy.Method: An event study methodology.Theory: Efficient market hypothesis, signal theory.Empirical results: Quantitative data from observations of stock prices in thirty two companies listed on the Nasdaq OMX Stockholm, in the period 2008 -2012.Conclusion: The results indicate that the market reaction was negative because there were negative abnormal returns on the Stockholm Stock Exchange during the period 2008-2012 in connection with the layoff announcements. The market reaction was milder when staff reductions were carried out as a result of a restructuring and stronger when staff reductions were carried out as part of a cost reduction program.The layoff announcements elicited different market reactions depending on the company's industry affiliation. The most negative reaction was in the Healthcare industry. Negative abnormal returns were lower in industries Industrials, Consumer Services and Consumer Goods. There were no abnormal returns for shares of companies in the industry Financials.

Dyskalkyli (specifika ra?knesva?righeter) a?r en av flera orsaker till matematiksva?righeter. Studier har pa?visat samband mellan dyskalkyli och dyslexi och att personer med dyskalkyli har sva?rt att klara skolmatematiken. Tva? skilda synsa?tt fo?rklarar orsaken till dyskalkyli: systemteorin och modula?rteorin.

This study was performed in order to evaluate if the method of collecting uterine secretion by the tampon-method is representative for use in studies of uterine secretions in mares. Uterine secretion collected by tampon in six mares was compared with secretion collected by aspiration, regarded to be a native sample, at the Institution of Obstetrics and Gynaecology, SLU, Uppsala. Proteinanalysis was made at the Department of Farmaceutic Bioscience, toxicological unit, Uppsala University. Cytological smears and ultrasonography was used to assess if the tampon had caused an inflammatoric reaction that could influence the protein content of the samples. The results suggest that the tampon and aspirat method do not differ when it comes to proteinconcentration, but the distribution of proteins differ between these different collection methods. This difference could not likely be explained by means of proteins due to an inflammatory reaction from the tampon, since neither the cytological nor the ultrasonographical examination gave any indications of inflammation.

The aim of this study is to examine how the public responds to the journalists? use of subjectivity in the reporting of broadcasting news. The investigation is a qualitative study based on nine interviews with respondents in ages of 21 to 25 from different parts of Sweden. After showing five examples of news reporting with a subjective approach, the respondents gave their look on the use of subjectivity. The theoretical frame of the investigation is founded on the theory of framing (de Vreese, 2005; Entman, 1993) and the discussion concerning the terms objectivity and subjectivity (e.g.